Antioxidant Enzyme Services (AES) to provide easy access to technologies for modifying and measuring molecules responsible for pro-oxidant formation, metabolism of reactive oxygen species, and mediators of redox biology including: anti-oxidant proteins/enzymes, small molecular weight cellular thiols and reductants, as well as redox mediated signaling and gene expression pathways governing growth, differentiation, and cell injury processes.
These services include molecular biology reagents to transfect and infect cells with adenovirus and plasmid vectors that cause over expression of anti-oxidant proteins, providing QA on the levels of expression and enzymatic activity of cells, and QA to ensure that the desired stable integration or transient expression of various proteins has occurred as well as RTPCR analysis of SNPs and other genes of interest in free radical biology. Resources available through the AES include antibodies, cDNAs, lentiviral vectors, cell lines, transgenic mice models, primers, and well characterized expression vectors. The AES works closely with other cores in this regard, including the Gene Transfer Vector Core and the Hybridoma Core.
AES Hours and Contact Information: Services are available 8AM-5PM Monday through Friday (and at other times based on specific discussion with Drs. Spitz). To set up an appointment, or if you have questions, please contact Douglas Spitz at douglas-spitz@uiowa.edu.
