Electron Spin Resonance (ESR) spectroscopy, as well as other detection methodologies, are available for measuring free radicals, singlet oxygen, nitric oxide, and the array of related oxidants and oxidative damage products. The ESR facility assists users in the detection of: free radicals in systems that range from solids, solutions, cells, tissues, and whole animals; nitric oxide and related metabolites including peroxynitrite; MDA/TBARS, indicators of lipid peroxidation that are detected with fluorescence spectroscopy; oxidation of fluorescent dyes as indicators of oxidation reactions in living cells; UV-Vis spectroscopy for kinetic studies of PhGPx (GPx4); cellular oxygen consumption for studying metabolic oxidations reactions; antioxidants such as vitamins C and E, beta-carotene; and precise cell volume measurements for quantitative redox biology.

ESR Hours and Contact Information:  Services are available 8AM-5PM Monday through Friday (and at other times based on specific discussion with Drs. Spitz and Buettner). To set up an appointment, or if you have questions, please contact: Brett Wagner at brett-wagner@uiowa.edu.