The Fundamental Chemistry and Biochemistry of Vitamins C and E

As part of this effort, we have developed two complementary assays for vitamin C that are very time- and cost-efficient that are being used to assess ascorbate levels in patient samples from clinical trials. 

Nitric Oxide

Nitric oxide is being investigated as an important membrane antioxidant in cells and tissue. We hypothesize that it is as important as Vitamin E.

Use of the Ascorbate Radical as a Marker for Free Radical Oxidative Stress

This research has provided the foundation for many ongoing free radical-related research projects here at Iowa and around the world.

Nitric Oxide Production During Stress

We are developing methods to monitor NO production in vivo. We have demonstrated that NO is produced during heat stress and we now extended this to local hyperthermia. We have extended this research into the world of hyperthermia as used in the treatment of cancer. We have already demonstrated that local hyperthermia of tumor tissue results in both free radical and NO production.

Redox state

This research effort has as a goal to arrive at a quantitative measurement of the redox environment of a cell/tissue.

Free Radicals in Membrane Lipid Peroxidation 

This ongoing theme addresses the fundamental mechanisms of how vitamin E protects cell membranes from oxidant stress. We have development methods to monitor lipid radical formation in real time from intact, functioning cells.

Photodynamic Cancer Therapy 

This research has been an important theme in the lab for many years. We have demonstrated mechanisms by which PDT produces free radicals in cells and tissues.

Free radicals in myocardial ischemia reperfusion and defibrillation injury

In this project we used ESR to observe the real-time production of free radicals associated with these stresses.  Importantly, approaches that can be used to blunt this injury were discovered.   

The Role of Metals in Free Radical Oxidations

This has been a long time area of research. We were the first to demonstrate that adventitious iron can change the results observed in free radical experiments and that chelating agents could be used to modulate the catalytic activity of metals. This work has been honored as a Citation Classic.

The Chemistry of EPR Spin Trapping

This represents the ongoing developmental aspects of the ESR Facility.

UV light and free radical production in skin.

Thus far, the results of this project are the first to demonstrate directly UV light production of radicals in skin and that antioxidants blunt this radical formation with a concomitant reduction of skin tumor formation.